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Addgene inc human dkc1 cdna

A: Western blot showing levels of dyskerin, NHP2 and NAF1 proteins in the iPS cells. All mutant cells show slightly decreased level of dyskerin protein compared to WT cells. Note that A353V iPS cells show decreased level of NHP2 while ΔL37 and Q31E cells don’t. The quantitive data, derived by densitometry, are shown. B: Real-time RT/PCR of RNA from iPS cells from DC patients and healthy controls. ΔL37 and Q31E mutant <t>DKC1</t> iPS cells express same amount of DKC1 mRNA as WT iPS cells, but A353V mutant iPS cells show a slightly decreased level of DKC1 mRNA. GAPDH was used at loading control. The combined results of 4 independent experiments are shown, the error bars show standard deviation. C: Real-Time RT/PCR of TERC RNA (left) and TERT mRNA (right) in iPS cells. GAPDH was used at loading control. The combined results of 3 independent experiments are shown, the error bars show standard deviation. * p<0.01 between WT cells and different mutant cells, respectively. D: Telomerase activity assay of ΔL37 (left) and A353V (right) iPS cells compared to WT cells by using TRAP assay. 2×10 6 cells were extracted by using CHAPS lysis buffer and serial dilutions (50ng, 10ng, 2ng and 0.4ng) of each sample were assayed. IC: internal control, HI: heat inactivation. The quantitive data, derived by densitometry, are shown. E: Telomere length measurement of the iPS cells in different passages compared to those from the original fibroblast cells (Fib) by using pulse field gel electrophoresis and in-gel hybridization with telomere probe (TTAGGG) 3 .

Human Dkc1 Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+dkc1+cdna/DKC1+(Plasmid+%23155548)/pmc04436374-92-15-13
Average 90 stars, based on 2 article reviews

human dkc1 cdna - by Bioz Stars, 2026-09

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1) Product Images from "Impaired Telomere Maintenance and Decreased Canonical WNT Signaling but Normal Ribosome Biogenesis in Induced Pluripotent Stem Cells from X-Linked Dyskeratosis Congenita Patients"

Article Title: Impaired Telomere Maintenance and Decreased Canonical WNT Signaling but Normal Ribosome Biogenesis in Induced Pluripotent Stem Cells from X-Linked Dyskeratosis Congenita Patients

Journal: PLoS ONE

doi: 10.1371/journal.pone.0127414

Figure Legend Snippet: A: Western blot showing levels of dyskerin, NHP2 and NAF1 proteins in the iPS cells. All mutant cells show slightly decreased level of dyskerin protein compared to WT cells. Note that A353V iPS cells show decreased level of NHP2 while ΔL37 and Q31E cells don’t. The quantitive data, derived by densitometry, are shown. B: Real-time RT/PCR of RNA from iPS cells from DC patients and healthy controls. ΔL37 and Q31E mutant DKC1 iPS cells express same amount of DKC1 mRNA as WT iPS cells, but A353V mutant iPS cells show a slightly decreased level of DKC1 mRNA. GAPDH was used at loading control. The combined results of 4 independent experiments are shown, the error bars show standard deviation. C: Real-Time RT/PCR of TERC RNA (left) and TERT mRNA (right) in iPS cells. GAPDH was used at loading control. The combined results of 3 independent experiments are shown, the error bars show standard deviation. * p<0.01 between WT cells and different mutant cells, respectively. D: Telomerase activity assay of ΔL37 (left) and A353V (right) iPS cells compared to WT cells by using TRAP assay. 2×10 6 cells were extracted by using CHAPS lysis buffer and serial dilutions (50ng, 10ng, 2ng and 0.4ng) of each sample were assayed. IC: internal control, HI: heat inactivation. The quantitive data, derived by densitometry, are shown. E: Telomere length measurement of the iPS cells in different passages compared to those from the original fibroblast cells (Fib) by using pulse field gel electrophoresis and in-gel hybridization with telomere probe (TTAGGG) 3 .

Techniques Used: Western Blot, Mutagenesis, Derivative Assay, Quantitative RT-PCR, Control, Standard Deviation, Telomerase Activity Assay, TRAP Assay, Lysis, Nucleic Acid Electrophoresis, Hybridization

A: measurement of pseudouridine in 28S rRNA from WT and mutant iPS cells. iPS cells were labeled with 32 P-labeled orthophosphate and 28S rRNA was gel-purified. After digestion with RNase T 2 , each sample was separated by two-dimensional TLC. The positions of the labeled ribonucleotides are indicated. Ap: Adenine, Cp: Cytosine, Gp: Guanine, Up: Uridine, Ψp: Pseudouridine: B: Pulse–chase labeling experiments of rRNA isolated from WT , A353V and ΔL37 iPS cells. Cells were labeled with L-[ 3 H-methyl] methionine for 30 min and then chased in nonradioactive medium for the times shown. The RNA was separated on a 1.25% agarose gel, transferred to a nylon filter, and exposed to x-ray film. C: Real-time RT/PCR results of some H/ACA snoRNAs of WT and DKC1 mutant iPS cells. Results were expressed relative to GAPDH RNA. The combined results of 3 independent experiments are shown, the error bars show standard deviation.

Figure Legend Snippet: A: measurement of pseudouridine in 28S rRNA from WT and mutant iPS cells. iPS cells were labeled with 32 P-labeled orthophosphate and 28S rRNA was gel-purified. After digestion with RNase T 2 , each sample was separated by two-dimensional TLC. The positions of the labeled ribonucleotides are indicated. Ap: Adenine, Cp: Cytosine, Gp: Guanine, Up: Uridine, Ψp: Pseudouridine: B: Pulse–chase labeling experiments of rRNA isolated from WT , A353V and ΔL37 iPS cells. Cells were labeled with L-[ 3 H-methyl] methionine for 30 min and then chased in nonradioactive medium for the times shown. The RNA was separated on a 1.25% agarose gel, transferred to a nylon filter, and exposed to x-ray film. C: Real-time RT/PCR results of some H/ACA snoRNAs of WT and DKC1 mutant iPS cells. Results were expressed relative to GAPDH RNA. The combined results of 3 independent experiments are shown, the error bars show standard deviation.

Techniques Used: Mutagenesis, Labeling, Purification, Pulse Chase, Isolation, Agarose Gel Electrophoresis, Quantitative RT-PCR, Standard Deviation

A: Schematic representation of zinc-finger nuclease (ZNF)-mediated homologous recombination. The constitutively active AAVS1 “safe harbor” locus is shown on the top line and the targeting construct is shown below. The cDNA expression cassettes driving expression of Flag-tagged WT dyskerin under the chicken actin promoter (CAGG) were inserted by zinc finger-mediated homologous recombination into intron 1 of AAVS1 . HA, homologous arms left (L) and right (R); SA-2APuro-PA, puromycin drug resistance cassette. B: Western blot showing expression of flag-tagged dyskerin protein in corrected A353V and ΔL37 iPS cells by using anti-Flag antibody and anti-dyskerin antibody. Open arrow: endogenous dyskerin protein; filled arrow: Flag-tagged WT dyskerin protein. C: Northern blot result of TERC RNA expression levels of DKC1 corrected iPS cells. Different corrected lines are shown as well as the uncorrected line carrying the DKC1 ΔL37 or the DKC1 A353V mutation. β-actin was used as loading control. The quantitive data, derived by densitometry, are shown. D: The TRAP assay was performed to measure the telomerase activity after expressing the WT DKC1 gene in A353V and ΔL37 iPS cells, uncorrected and corrected by ectopic expression of WT DKC1 (DKC1-C) . The quantitive data, derived by densitometry, are shown E: Telomere lengths of DKC1 corrected iPS cells were measured by using in-gel hybridization with a telomere probe (TTAGGG) 3 . DKC1-C indicates corrected iPS cells expressing WT DKC1 gene.

Figure Legend Snippet: A: Schematic representation of zinc-finger nuclease (ZNF)-mediated homologous recombination. The constitutively active AAVS1 “safe harbor” locus is shown on the top line and the targeting construct is shown below. The cDNA expression cassettes driving expression of Flag-tagged WT dyskerin under the chicken actin promoter (CAGG) were inserted by zinc finger-mediated homologous recombination into intron 1 of AAVS1 . HA, homologous arms left (L) and right (R); SA-2APuro-PA, puromycin drug resistance cassette. B: Western blot showing expression of flag-tagged dyskerin protein in corrected A353V and ΔL37 iPS cells by using anti-Flag antibody and anti-dyskerin antibody. Open arrow: endogenous dyskerin protein; filled arrow: Flag-tagged WT dyskerin protein. C: Northern blot result of TERC RNA expression levels of DKC1 corrected iPS cells. Different corrected lines are shown as well as the uncorrected line carrying the DKC1 ΔL37 or the DKC1 A353V mutation. β-actin was used as loading control. The quantitive data, derived by densitometry, are shown. D: The TRAP assay was performed to measure the telomerase activity after expressing the WT DKC1 gene in A353V and ΔL37 iPS cells, uncorrected and corrected by ectopic expression of WT DKC1 (DKC1-C) . The quantitive data, derived by densitometry, are shown E: Telomere lengths of DKC1 corrected iPS cells were measured by using in-gel hybridization with a telomere probe (TTAGGG) 3 . DKC1-C indicates corrected iPS cells expressing WT DKC1 gene.

Techniques Used: Zinc-Fingers, Homologous Recombination, Construct, Expressing, Western Blot, Northern Blot, RNA Expression, Mutagenesis, Control, Derivative Assay, TRAP Assay, Activity Assay, Hybridization

Figure Legend Snippet: Fold changes of WNT related genes in DKC1 mutant iPS cells.

Techniques Used: Mutagenesis

A: Validation of the results from microarray. LGR5 , DKK1 , WLS and FRZB mRNA expression were measured by real-time RT/PCR. 3 independent experiments were performed and the error bars show standard deviation. TERT CP: iPS cells with TERT R537H/2173-2187del15insACAG compound homozygotes mutation * p<0.01 between WT iPS cells and different mutant iPS cells, respectively. B: Western blot result of dyskerin protein in HEK293T cells showing doxycycline inducible DKC1 shRNA can reduce the dyskerin level to about 10–20%. shGFP was used as a control. C: Telomerase activity of HEK293T cells after knocking down of dyskerin protein from B was measured by TRAP assay. The quantitive data, derived by densitometry, are shown. D; Knock down of dyskerin protein can directly decrease the expression of LGR5 , WLS and FRZB genes. Two different DKC1 shRNA lines were used in this experiment. The combined results of 3 independent experiments are shown, the error bars show standard deviation. * p<0.01.

Figure Legend Snippet: A: Validation of the results from microarray. LGR5 , DKK1 , WLS and FRZB mRNA expression were measured by real-time RT/PCR. 3 independent experiments were performed and the error bars show standard deviation. TERT CP: iPS cells with TERT R537H/2173-2187del15insACAG compound homozygotes mutation * p<0.01 between WT iPS cells and different mutant iPS cells, respectively. B: Western blot result of dyskerin protein in HEK293T cells showing doxycycline inducible DKC1 shRNA can reduce the dyskerin level to about 10–20%. shGFP was used as a control. C: Telomerase activity of HEK293T cells after knocking down of dyskerin protein from B was measured by TRAP assay. The quantitive data, derived by densitometry, are shown. D; Knock down of dyskerin protein can directly decrease the expression of LGR5 , WLS and FRZB genes. Two different DKC1 shRNA lines were used in this experiment. The combined results of 3 independent experiments are shown, the error bars show standard deviation. * p<0.01.

Techniques Used: Biomarker Discovery, Microarray, Expressing, Quantitative RT-PCR, Standard Deviation, Mutagenesis, Western Blot, shRNA, Control, Activity Assay, TRAP Assay, Derivative Assay, Knockdown

A: Real-Time RT/PCR experiments showed that, in A353V iPS cells, the mRNA expression of LGR5 , FRZB and WLS was significantly increased after expressing WT dyskerin protein. The combined results of 3 independent experiments are shown, the error bars show standard deviation. * p<0.01. B: Knock down of dyskerin affects canonical WNT signaling. The relative luciferase activity in inducible DKC1 -shRNA HEK293T cells transfected with plasmids containing a luciferase gene under the control of a WT (OT-flash) or mutant (OF-flash) β-catenin responsive promotor and pRL-SV40 plasmid. Luciferase activity was measured by using dual-luciferase system according to protocol from manufacturer. OT: WT TCF/LEF reporter, OF: Mutant TCF/LEF reporter, Dox: Doxycycline, CHIR: CHIR-99021.Four independent experiments were carried out and the mean of results are shown; the error bars showing standard deviation. * p<0.01.

Figure Legend Snippet: A: Real-Time RT/PCR experiments showed that, in A353V iPS cells, the mRNA expression of LGR5 , FRZB and WLS was significantly increased after expressing WT dyskerin protein. The combined results of 3 independent experiments are shown, the error bars show standard deviation. * p<0.01. B: Knock down of dyskerin affects canonical WNT signaling. The relative luciferase activity in inducible DKC1 -shRNA HEK293T cells transfected with plasmids containing a luciferase gene under the control of a WT (OT-flash) or mutant (OF-flash) β-catenin responsive promotor and pRL-SV40 plasmid. Luciferase activity was measured by using dual-luciferase system according to protocol from manufacturer. OT: WT TCF/LEF reporter, OF: Mutant TCF/LEF reporter, Dox: Doxycycline, CHIR: CHIR-99021.Four independent experiments were carried out and the mean of results are shown; the error bars showing standard deviation. * p<0.01.

Techniques Used: Quantitative RT-PCR, Expressing, Standard Deviation, Knockdown, Luciferase, Activity Assay, shRNA, Transfection, Control, Mutagenesis, Plasmid Preparation

Construct:

Article Title: Impaired Telomere Maintenance and Decreased Canonical WNT Signaling but Normal Ribosome Biogenesis in Induced Pluripotent Stem Cells from X-Linked Dyskeratosis Congenita Patients
Article Snippet: .. The donor construct was targeted to the AAVS1 locus using the AAVS1-SA-2A-puro-pA plasmid (Addgene) containing human DKC1 cDNA driven by the chicken β actin promoter. .. Approximately 1x10 5 iPSCs were plated onto puromycin-resistant MEF feeder cells with 10 μM Rock inhibitor (Cayman Chemical, Ann Arbor, MI, USA), then transfected using X-tremeGENE 9 (Roche, Indianapolis, IN, USA).

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Journal: PLoS ONE

Article Title: Impaired Telomere Maintenance and Decreased Canonical WNT Signaling but Normal Ribosome Biogenesis in Induced Pluripotent Stem Cells from X-Linked Dyskeratosis Congenita Patients

doi: 10.1371/journal.pone.0127414

Figure Lengend Snippet: A: Western blot showing levels of dyskerin, NHP2 and NAF1 proteins in the iPS cells. All mutant cells show slightly decreased level of dyskerin protein compared to WT cells. Note that A353V iPS cells show decreased level of NHP2 while ΔL37 and Q31E cells don’t. The quantitive data, derived by densitometry, are shown. B: Real-time RT/PCR of RNA from iPS cells from DC patients and healthy controls. ΔL37 and Q31E mutant DKC1 iPS cells express same amount of DKC1 mRNA as WT iPS cells, but A353V mutant iPS cells show a slightly decreased level of DKC1 mRNA. GAPDH was used at loading control. The combined results of 4 independent experiments are shown, the error bars show standard deviation. C: Real-Time RT/PCR of TERC RNA (left) and TERT mRNA (right) in iPS cells. GAPDH was used at loading control. The combined results of 3 independent experiments are shown, the error bars show standard deviation. * p<0.01 between WT cells and different mutant cells, respectively. D: Telomerase activity assay of ΔL37 (left) and A353V (right) iPS cells compared to WT cells by using TRAP assay. 2×10 6 cells were extracted by using CHAPS lysis buffer and serial dilutions (50ng, 10ng, 2ng and 0.4ng) of each sample were assayed. IC: internal control, HI: heat inactivation. The quantitive data, derived by densitometry, are shown. E: Telomere length measurement of the iPS cells in different passages compared to those from the original fibroblast cells (Fib) by using pulse field gel electrophoresis and in-gel hybridization with telomere probe (TTAGGG) 3 .

Article Snippet: The donor construct was targeted to the AAVS1 locus using the AAVS1-SA-2A-puro-pA plasmid (Addgene) containing human DKC1 cDNA driven by the chicken β actin promoter.

Techniques: Western Blot, Mutagenesis, Derivative Assay, Quantitative RT-PCR, Control, Standard Deviation, Telomerase Activity Assay, TRAP Assay, Lysis, Nucleic Acid Electrophoresis, Hybridization

Journal: PLoS ONE

Article Title: Impaired Telomere Maintenance and Decreased Canonical WNT Signaling but Normal Ribosome Biogenesis in Induced Pluripotent Stem Cells from X-Linked Dyskeratosis Congenita Patients

doi: 10.1371/journal.pone.0127414

Figure Lengend Snippet: A: measurement of pseudouridine in 28S rRNA from WT and mutant iPS cells. iPS cells were labeled with 32 P-labeled orthophosphate and 28S rRNA was gel-purified. After digestion with RNase T 2 , each sample was separated by two-dimensional TLC. The positions of the labeled ribonucleotides are indicated. Ap: Adenine, Cp: Cytosine, Gp: Guanine, Up: Uridine, Ψp: Pseudouridine: B: Pulse–chase labeling experiments of rRNA isolated from WT , A353V and ΔL37 iPS cells. Cells were labeled with L-[ 3 H-methyl] methionine for 30 min and then chased in nonradioactive medium for the times shown. The RNA was separated on a 1.25% agarose gel, transferred to a nylon filter, and exposed to x-ray film. C: Real-time RT/PCR results of some H/ACA snoRNAs of WT and DKC1 mutant iPS cells. Results were expressed relative to GAPDH RNA. The combined results of 3 independent experiments are shown, the error bars show standard deviation.

Article Snippet: The donor construct was targeted to the AAVS1 locus using the AAVS1-SA-2A-puro-pA plasmid (Addgene) containing human DKC1 cDNA driven by the chicken β actin promoter.

Techniques: Mutagenesis, Labeling, Purification, Pulse Chase, Isolation, Agarose Gel Electrophoresis, Quantitative RT-PCR, Standard Deviation

Journal: PLoS ONE

Article Title: Impaired Telomere Maintenance and Decreased Canonical WNT Signaling but Normal Ribosome Biogenesis in Induced Pluripotent Stem Cells from X-Linked Dyskeratosis Congenita Patients

doi: 10.1371/journal.pone.0127414

Figure Lengend Snippet: A: Schematic representation of zinc-finger nuclease (ZNF)-mediated homologous recombination. The constitutively active AAVS1 “safe harbor” locus is shown on the top line and the targeting construct is shown below. The cDNA expression cassettes driving expression of Flag-tagged WT dyskerin under the chicken actin promoter (CAGG) were inserted by zinc finger-mediated homologous recombination into intron 1 of AAVS1 . HA, homologous arms left (L) and right (R); SA-2APuro-PA, puromycin drug resistance cassette. B: Western blot showing expression of flag-tagged dyskerin protein in corrected A353V and ΔL37 iPS cells by using anti-Flag antibody and anti-dyskerin antibody. Open arrow: endogenous dyskerin protein; filled arrow: Flag-tagged WT dyskerin protein. C: Northern blot result of TERC RNA expression levels of DKC1 corrected iPS cells. Different corrected lines are shown as well as the uncorrected line carrying the DKC1 ΔL37 or the DKC1 A353V mutation. β-actin was used as loading control. The quantitive data, derived by densitometry, are shown. D: The TRAP assay was performed to measure the telomerase activity after expressing the WT DKC1 gene in A353V and ΔL37 iPS cells, uncorrected and corrected by ectopic expression of WT DKC1 (DKC1-C) . The quantitive data, derived by densitometry, are shown E: Telomere lengths of DKC1 corrected iPS cells were measured by using in-gel hybridization with a telomere probe (TTAGGG) 3 . DKC1-C indicates corrected iPS cells expressing WT DKC1 gene.

Article Snippet: The donor construct was targeted to the AAVS1 locus using the AAVS1-SA-2A-puro-pA plasmid (Addgene) containing human DKC1 cDNA driven by the chicken β actin promoter.

Techniques: Zinc-Fingers, Homologous Recombination, Construct, Expressing, Western Blot, Northern Blot, RNA Expression, Mutagenesis, Control, Derivative Assay, TRAP Assay, Activity Assay, Hybridization

Journal: PLoS ONE

Article Title: Impaired Telomere Maintenance and Decreased Canonical WNT Signaling but Normal Ribosome Biogenesis in Induced Pluripotent Stem Cells from X-Linked Dyskeratosis Congenita Patients

doi: 10.1371/journal.pone.0127414

Figure Lengend Snippet: Fold changes of WNT related genes in DKC1 mutant iPS cells.

Article Snippet: The donor construct was targeted to the AAVS1 locus using the AAVS1-SA-2A-puro-pA plasmid (Addgene) containing human DKC1 cDNA driven by the chicken β actin promoter.

Techniques: Mutagenesis

Journal: PLoS ONE

Article Title: Impaired Telomere Maintenance and Decreased Canonical WNT Signaling but Normal Ribosome Biogenesis in Induced Pluripotent Stem Cells from X-Linked Dyskeratosis Congenita Patients

doi: 10.1371/journal.pone.0127414

Figure Lengend Snippet: A: Validation of the results from microarray. LGR5 , DKK1 , WLS and FRZB mRNA expression were measured by real-time RT/PCR. 3 independent experiments were performed and the error bars show standard deviation. TERT CP: iPS cells with TERT R537H/2173-2187del15insACAG compound homozygotes mutation * p<0.01 between WT iPS cells and different mutant iPS cells, respectively. B: Western blot result of dyskerin protein in HEK293T cells showing doxycycline inducible DKC1 shRNA can reduce the dyskerin level to about 10–20%. shGFP was used as a control. C: Telomerase activity of HEK293T cells after knocking down of dyskerin protein from B was measured by TRAP assay. The quantitive data, derived by densitometry, are shown. D; Knock down of dyskerin protein can directly decrease the expression of LGR5 , WLS and FRZB genes. Two different DKC1 shRNA lines were used in this experiment. The combined results of 3 independent experiments are shown, the error bars show standard deviation. * p<0.01.

Article Snippet: The donor construct was targeted to the AAVS1 locus using the AAVS1-SA-2A-puro-pA plasmid (Addgene) containing human DKC1 cDNA driven by the chicken β actin promoter.

Techniques: Biomarker Discovery, Microarray, Expressing, Quantitative RT-PCR, Standard Deviation, Mutagenesis, Western Blot, shRNA, Control, Activity Assay, TRAP Assay, Derivative Assay, Knockdown

Journal: PLoS ONE

Article Title: Impaired Telomere Maintenance and Decreased Canonical WNT Signaling but Normal Ribosome Biogenesis in Induced Pluripotent Stem Cells from X-Linked Dyskeratosis Congenita Patients

doi: 10.1371/journal.pone.0127414

Figure Lengend Snippet: A: Real-Time RT/PCR experiments showed that, in A353V iPS cells, the mRNA expression of LGR5 , FRZB and WLS was significantly increased after expressing WT dyskerin protein. The combined results of 3 independent experiments are shown, the error bars show standard deviation. * p<0.01. B: Knock down of dyskerin affects canonical WNT signaling. The relative luciferase activity in inducible DKC1 -shRNA HEK293T cells transfected with plasmids containing a luciferase gene under the control of a WT (OT-flash) or mutant (OF-flash) β-catenin responsive promotor and pRL-SV40 plasmid. Luciferase activity was measured by using dual-luciferase system according to protocol from manufacturer. OT: WT TCF/LEF reporter, OF: Mutant TCF/LEF reporter, Dox: Doxycycline, CHIR: CHIR-99021.Four independent experiments were carried out and the mean of results are shown; the error bars showing standard deviation. * p<0.01.

Article Snippet: The donor construct was targeted to the AAVS1 locus using the AAVS1-SA-2A-puro-pA plasmid (Addgene) containing human DKC1 cDNA driven by the chicken β actin promoter.

Techniques: Quantitative RT-PCR, Expressing, Standard Deviation, Knockdown, Luciferase, Activity Assay, shRNA, Transfection, Control, Mutagenesis, Plasmid Preparation

Figure 2 | Telomere elongation in DKC1 mutant iPS cells. a, Telomere Southern blot of del37L fibroblasts (Fib) and iPS clones 1 and 2 as a function of passage. MM, molecular mass marker. b, Quantitative real-time PCR (qPCR) analysis of telomere length in del37L fibroblasts and iPS clones at indicated passages (p). Error bars represent s.e.m. c, Quantitative fluorescence in situ hybridization (qFISH) for telomere length in del37L cells. a.f.u., arbitrary fluorescence units. Inset, mean relative length values shown 6s.d.

Journal: Nature

Article Title: Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patients.

doi: 10.1038/nature08792

Figure Lengend Snippet: Figure 2 | Telomere elongation in DKC1 mutant iPS cells. a, Telomere Southern blot of del37L fibroblasts (Fib) and iPS clones 1 and 2 as a function of passage. MM, molecular mass marker. b, Quantitative real-time PCR (qPCR) analysis of telomere length in del37L fibroblasts and iPS clones at indicated passages (p). Error bars represent s.e.m. c, Quantitative fluorescence in situ hybridization (qFISH) for telomere length in del37L cells. a.f.u., arbitrary fluorescence units. Inset, mean relative length values shown 6s.d.

Article Snippet: The DKC1 retroviral vector was created by cloning a full-length DKC1 cDNA (OriGene) into pMIG-W (Addgene) and retrovirus was produced as described31.

Techniques: Mutagenesis, Southern Blot, Clone Assay, Marker, Real-time Polymerase Chain Reaction, Fluorescence, In Situ Hybridization

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human dkc1 cdna (Addgene inc)

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1) Product Images from "Impaired Telomere Maintenance and Decreased Canonical WNT Signaling but Normal Ribosome Biogenesis in Induced Pluripotent Stem Cells from X-Linked Dyskeratosis Congenita Patients"

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